Recombinant Cystatin-Like Thiol Proteinase Inhibitor (rCTPI) of Cicer arietinum (Chickpea): A potential source of Bio-pesticide.

Madhu v, Vineet Kumar, Ajay Kumar, Yagyavallcya Sharma, Navin Kumar Ojha


Cystatins are thiol proteinase inhibitors ubiquitously present in many plants as well as animal tissues. In the present study, a cystatin like a thiol proteinase inhibitor (rCTPI) gene was isolated from chickpea plant and cloned in pET28a vector. The recombinant vector with CTPI gene was expressed and recombinant CTPI proteins was isolated and purified. The purified rCTPI was physiologically active at pH 7.5 and temperature range of 35-370 C. Larvicidal activity of rCTPI were determined with a potential application as bio-pesticide.

Full Text:



  • There are currently no refbacks.

Copyright (c) 2019 Edupedia Publications Pvt Ltd

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


EduPedia Publications Pvt Ltd, D-351, Prem Nagar-2, Suleman Nagar, Kirari, Nagloi, New Delhi PIN-Code 110086, India Through Phone Call us now: +919958037887 or +919557022047

All published Articles are Open Access at

Paper submission: or


Mobile:                  +919557022047 & +919958037887


Journals Maintained and Hosted by

EduPedia Publications (P) Ltd in Association with Other Institutional Partners

Pen2Print and IJR are registered trademark of the Edupedia Publications Pvt Ltd.